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用于估计恒河猴(猕猴属)亲缘关系的新增高度多态性微卫星(STR)位点。

Additional highly polymorphic microsatellite (STR) loci for estimating kinship in rhesus macaques (Macaca mulatta).

作者信息

Smith D G, Kanthaswamy S, Viray J, Cody L

机构信息

Department of Anthropology, California Regional Primate Research Center, University of California, Davis 95616, USA.

出版信息

Am J Primatol. 2000 Jan;50(1):1-7. doi: 10.1002/(SICI)1098-2345(200001)50:1<1::AID-AJP1>3.0.CO;2-T.

Abstract

Thirty-four short tandem repeat (STR) loci, not previously studied in rhesus macaques, were amplified by PCR. About one third of these were found to clearly and reliably amplify and exhibit high levels of genetic heterogeneity even in relatively inbred populations. These loci, together with 11 loci previously studied, were sufficiently informative to discretely differentiate between related and unrelated pairs and, in most cases, between parent/offspring and other relative pairs. An even greater number of hypervariable STR loci might be required to distinguish between half-sib and full-sib pairs in most rhesus populations.

摘要

通过聚合酶链反应(PCR)扩增了34个短串联重复序列(STR)位点,这些位点此前未在恒河猴中进行过研究。结果发现,其中约三分之一的位点能够清晰且可靠地扩增,并且即使在相对近亲繁殖的群体中也表现出高度的遗传异质性。这些位点与之前研究的11个位点一起,信息量足以在亲缘关系和非亲缘关系个体之间,以及在大多数情况下,在亲子和其他亲属关系个体之间进行明确区分。在大多数恒河猴群体中,可能需要更多数量的高变STR位点来区分半同胞和全同胞个体。

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