嘌呤能P2X受体在大鼠心脏中的分布。

Distribution of purinergic P2X receptors in the rat heart.

作者信息

Hansen M A, Bennett M R, Barden J A

机构信息

The Institute for Biomedical Research, The University of Sydney, NSW, Australia.

出版信息

J Auton Nerv Syst. 1999 Oct 8;78(1):1-9. doi: 10.1016/s0165-1838(99)00046-6.

DOI:10.1016/s0165-1838(99)00046-6

PMID:10589817

Abstract

The distribution of P2X purinergic receptor subtypes has been determined in relation to nerve varicosities in the rat heart with immunohistochemistry. Large clusters (about 1 microm diameter) of co-localised and sometimes co-extensive P2X1 and P2X3 receptors were found at sites of tyrosine hydroxylase (TH) positive axon varicosities in the atrium and the ventricle. Varicosities that were labelled with antibodies to the synaptic vesicle epitope SV2 were frequently labelled also with antibodies to P2X3, P2X5 and P2X6 but not always with antibodies to P2X1. Especially prominent were large numbers of small clusters (about 400 nm diameter) of co-localised P2X2 and P2X5 receptors on the sarcolemma unrelated to nerves at all. During development the 1 day-old heart possessed an abundance of co-localised P2X2 and P2X5 small receptor clusters on the sarcolemma. These observations are discussed in relation to the role of purinergic receptors in the mammalian heart.

摘要

利用免疫组织化学方法，已确定大鼠心脏中P2X嘌呤能受体亚型与神经膨体的分布关系。在心房和心室中，酪氨酸羟化酶（TH）阳性轴突膨体部位发现了大量共定位且有时相互重叠的P2X1和P2X3受体簇（直径约1微米）。用针对突触囊泡表位SV2的抗体标记的膨体，也经常能用针对P2X3、P2X5和P2X6的抗体标记，但用针对P2X1的抗体标记时并非总是如此。特别显著的是，在与神经毫无关系的肌膜上有大量共定位的P2X2和P2X5受体小簇（直径约400纳米）。在发育过程中，1日龄的心脏在肌膜上有大量共定位的P2X2和P2X5小受体簇。本文结合嘌呤能受体在哺乳动物心脏中的作用对这些观察结果进行了讨论。