Afework M, Burnstock G
Department of Anatomy, Faculty of Medicine, Addis Ababa University, Ethiopia.
Cell Tissue Res. 1999 Dec;298(3):449-56. doi: 10.1007/s004419900103.
The distribution of each of the seven subtypes of ATP-gated P2X receptors was investigated in the adrenal gland of rat utilizing immunohistochemical techniques with specific polyclonal antibodies to unique peptide sequences of P2X1-7 receptors. A small number of chromaffin cells showed positive immunoreaction for P2X5 and P2X7, with the relative occurrence of P2X7-immunoreactive chromaffin cells exceeding that of P2X5. The preganglionic nerve fibres that form terminal plexuses around some chromaffin cells showed P2X1 immunoreactivity. Intrinsic adrenal neurones were observed to be positively stained for P2X2 and P2X3 receptors. P2X2 immunoreactivity occurred in several neurones found singly or in groups in the medulla, while only a small number of neurones were immunoreactive for P2X3. Adrenal cortical cells were positively immunostained for P2X4-7. Immunoreactivity for P2X4 was confined to the cells of the zona reticularis, while P2X5-7 immunoreactivities occurred in cells of the zona fasciculata. The relative occurrence of immunoreactive cortical cells of the zona fasciculata was highest for P2X6, followed by P2X7 and then P2X5. The smooth muscle of some capsular and subcapsular blood vessels showed P2X2 immunoreactivity. The specific and widespread distribution of P2X receptor subtypes in the adrenal gland suggests a significant role for purine signalling in the physiology of the rat adrenal gland.
利用针对P2X1 - 7受体独特肽序列的特异性多克隆抗体，采用免疫组织化学技术，研究了大鼠肾上腺中七种ATP门控P2X受体亚型各自的分布情况。少数嗜铬细胞对P2X5和P2X7呈阳性免疫反应，P2X7免疫反应性嗜铬细胞的相对发生率超过P2X5。在一些嗜铬细胞周围形成终末丛的节前神经纤维显示出P2X1免疫反应性。观察到肾上腺固有神经元对P2X2和P2X3受体呈阳性染色。P2X2免疫反应性出现在髓质中单个或成群的几个神经元中，而只有少数神经元对P2X3呈免疫反应性。肾上腺皮质细胞对P2X4 - 7呈阳性免疫染色。P2X4的免疫反应性局限于网状带细胞，而P2X5 - 7的免疫反应性出现在束状带细胞中。束状带免疫反应性皮质细胞的相对发生率以P2X6最高，其次是P2X7，然后是P2X5。一些被膜和被膜下血管的平滑肌显示出P2X2免疫反应性。P2X受体亚型在肾上腺中的特异性和广泛分布表明嘌呤信号在大鼠肾上腺生理学中具有重要作用。
