最小禽白血病-肉瘤病毒包装信号的二级结构分析
Secondary structure analysis of a minimal avian leukosis-sarcoma virus packaging signal.
作者信息
Banks J D, Linial M L
机构信息
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
出版信息
J Virol. 2000 Jan;74(1):456-64. doi: 10.1128/jvi.74.1.456-464.2000.
Abstract
We previously identified a 160-nucleotide packaging signal, MPsi, from the 5' end of the Rous sarcoma virus genome. In this study, we determine the secondary structure of MPsi by using phylogenetic analysis with computer modeling and heterologous packaging assays of point mutants. The results of the in vivo studies are in good agreement with the computer model. Additionally, the packaging studies indicate several structures which are important for efficient packaging, including a single-stranded bulge containing the initiation codon for the short open reading frame, uORF3, as well as adjacent stem structures. Finally, we show that the L3 stem-loop at the 3' end of MPsi is dispensable for packaging, thus identifying an 82-nucleotide minimal packaging signal, microPsi, composed of the O3 stem-loop.
摘要
我们之前从劳氏肉瘤病毒基因组的5'端鉴定出一个160个核苷酸的包装信号MPsi。在本研究中，我们通过计算机建模的系统发育分析和点突变体的异源包装试验来确定MPsi的二级结构。体内研究结果与计算机模型高度吻合。此外，包装研究表明有几个结构对有效包装很重要，包括一个包含短开放阅读框uORF3起始密码子的单链凸起以及相邻的茎结构。最后，我们表明MPsi 3'端的L3茎环对于包装是可有可无的，从而确定了一个由O3茎环组成的82个核苷酸的最小包装信号microPsi。
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本文引用的文献
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An Mpsi-containing heterologous RNA, but not env mRNA, is efficiently packaged into avian retroviral particles.
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