最小的禽逆转录病毒包装序列具有复杂的结构。
A minimal avian retroviral packaging sequence has a complex structure.
作者信息
Banks J D, Yeo A, Green K, Cepeda F, Linial M L
机构信息
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
出版信息
J Virol. 1998 Jul;72(7):6190-4. doi: 10.1128/JVI.72.7.6190-6194.1998.
Abstract
We have defined a 160-nucleotide region, Mpsi, from the 5' leader region of the Rous sarcoma virus genome that is sufficient to direct the packaging of a heterologous RNA. Mpsi contains the putative O3 stem structure that has previously been shown, and that has been confirmed in this study, to be important for the efficient packaging of avian leukosis-sarcoma virus RNA. Analyses of several O3 stem mutants revealed that other regions within Mpsi can interfere with the proper folding of altered sequences which are predicted to form a wild-type O3 stem.
摘要
我们从劳氏肉瘤病毒基因组的5'前导区定义了一个160个核苷酸的区域Mpsi，该区域足以指导异源RNA的包装。Mpsi包含推定的O3茎结构，此前已证明该结构对禽白血病-肉瘤病毒RNA的有效包装很重要，本研究也证实了这一点。对几个O3茎突变体的分析表明，Mpsi内的其他区域会干扰预测形成野生型O3茎的改变序列的正确折叠。
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