Sartorelli P, Cenni A, Matteucci G, Montomoli L, Novelli M T, Palmi S
Istituto di Medicina del Lavoro, Università degli Studi di Siena, Via dei Tufi 1, I-53100 Siena, Italy.
Int Arch Occup Environ Health. 1999 Nov;72(8):528-32. doi: 10.1007/s004200050411.
Percutaneous penetration of polycyclic aromatic hydrocarbons (PAHs) is affected by various factors connected to exposure conditions. The nature of the matrix, such as that of oil, can strongly affect their percutaneous penetration. Risk assessment should consider these effects. We examined the effect of matrix on percutaneous penetration of PAHs, particularly that of lubricating oil.
The test apparatus consisted of an in vitro static diffusion cell system using full-thickness monkey (Cercopithecus aetiops) skin as the membrane and saline solution with gentamycin sulfate and 4% bovine serum albumin as receptor fluid. Chemical analysis of PAHs in the samples obtained from cells was carried out by inverse-phase HPCL, and the results were read by spectrofluorimetry.
Comparing the penetration of 13 PAHs from a lubricating oil and from acetone solution with artificial sweat resulted in a significantly slower passage from the oil matrix for acenaphthene, anthracene, phenanthrene, fluoranthene, naphthalene, pyrene, fluorene (Mann-Whitney U test, P < 0.05). No significant differences in the passage were found for chrysene because, in the test with oil, its concentration was very often below the detection limit. For benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, and benzo[a]pyrene it was possible to demonstrate a passage through the skin only when compounds were applied in acetone solution with artificial sweat.
The results of the study suggest the necessity of dermal penetration data relevant for risk assessment, obtained under experimental conditions similar to the real exposure conditions.
多环芳烃（PAHs）的经皮渗透受与暴露条件相关的多种因素影响。基质的性质，如油的性质，会强烈影响其经皮渗透。风险评估应考虑这些影响。我们研究了基质对PAHs经皮渗透的影响，特别是润滑油的影响。
测试装置由体外静态扩散池系统组成，使用全层猴（非洲绿猴）皮肤作为膜，以含有硫酸庆大霉素和4%牛血清白蛋白的盐溶液作为接收液。通过反相高效液相色谱法对从池中获得的样品中的PAHs进行化学分析，并通过荧光光谱法读取结果。
比较13种PAHs从润滑油和含人工汗液的丙酮溶液中的渗透情况，苊、蒽、菲、荧蒽、萘、芘、芴从油基质中的渗透明显较慢（曼-惠特尼U检验，P<0.05）。对于屈，未发现渗透有显著差异，因为在油的测试中，其浓度经常低于检测限。对于苯并[a]蒽、苯并[b]荧蒽、苯并[k]荧蒽和苯并[a]芘，只有当化合物以含人工汗液的丙酮溶液形式应用时，才有可能证明其透过皮肤。
研究结果表明，有必要在与实际暴露条件相似的实验条件下获得与风险评估相关的皮肤渗透数据。
