Chow L, Yueh Y Y, Tseng W L, Wu Y C, Wu S C, Horng C B
National Institute of Preventive Medicine, Department of Health, Executive Yuan, Taipei, Taiwan, R.O.C.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1997 Nov;30(4):255-64.
Competitive ELISA was used for the detection of neutralizing antibody to JE. Based on the principle that human serum JE antibody competed with JE monoclonal antibody (MAb) for JE antigen, it was found that 3 JE MAbs (E3-3, NPF-5 and NNN-5) were suitable for competitive ELISA for the detection of JE neutralizing antibody. The sensitivity of cometitive ELISA for 29 JE confirmed serum specimens with titer of plaque reduction neutralization test (PRNT) was checked to be 82.1% (23/28). The specificity of E3-3 MAb to JE used in competitive ELISA was 100%. Correlation coefficient of JE confirmed cases of 57 hemagglutination inhibition (HI) titers in 1995 and 37 PRNT titers in 1994 compared with competitive ELISA were 0.744 and 0.732, respectively. Compared the competitive ELISA titers of 154 sera of healthy people with PRNT titers, the results showed that 70% of the sera could be detected by competitive ELISA which saved a lot of time and manpower.
采用竞争ELISA法检测乙脑中和抗体。基于人血清乙脑抗体与乙脑单克隆抗体（MAb）竞争乙脑抗原的原理，发现3株乙脑MAb（E3-3、NPF-5和NNN-5）适用于竞争ELISA法检测乙脑中和抗体。对29份经蚀斑减少中和试验（PRNT）确证的乙脑血清标本进行竞争ELISA法检测，其敏感性为82.1%（23/28）。竞争ELISA法中所用E3-3 MAb对乙脑的特异性为100%。1995年57份血凝抑制（HI）效价和1994年37份PRNT效价的乙脑确诊病例与竞争ELISA法比较的相关系数分别为0.744和0.732。将154份健康人血清的竞争ELISA效价与PRNT效价进行比较，结果显示竞争ELISA法可检测70%的血清，节省了大量时间和人力。
