Wang H, Le Dall M T, Waché Y, Laroche C, Belin J M, Nicaud J M
Laboratoire de Génétique des Microorganismes, INRA-CNRS, Centre de Biotechnologie Agro Industriel, INA-PG, Thiverval Grignon, France.
Cell Biochem Biophys. 1999;31(2):165-74. doi: 10.1007/BF02738170.
The Acyl-CoA oxidase (AOX) isozymes catalyze the first steps of peroxisomal beta-oxidation, which is important for the degradation of fatty acids. Using conserved blocks in previously identified yeast POX genes encoding AOXs, the authors have shown that five POX genes are present in the yeast Yarrowia lipolytica. These genes show approx 63% identity among themselves, and 42% identity with the POX genes from other yeasts. Mono-disrupted Y. lipolytica strains were constructed using a variation of the sticky-end polymerase chain reaction method. AOX activity in the mono-disrupted strains revealed that a long-chain oxidase is encoded by the POX2 gene and a short-chain oxidase by the POX3 gene.
酰基辅酶A氧化酶（AOX）同工酶催化过氧化物酶体β-氧化的起始步骤，这对脂肪酸的降解很重要。作者利用先前鉴定的编码AOX的酵母POX基因中的保守区域，发现解脂耶氏酵母中存在五个POX基因。这些基因彼此间显示出约63%的同源性，与其他酵母的POX基因有42%的同源性。利用粘性末端聚合酶链反应方法的一种变体构建了解脂耶氏酵母单基因破坏菌株。单基因破坏菌株中的AOX活性表明，POX2基因编码一种长链氧化酶，POX3基因编码一种短链氧化酶。
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