DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois At Urbana-Champaign, Urbana, IL, USA.
Department of Chemical and Biomolecular Engineering, University of Illinois At Urbana-Champaign, Urbana, IL, USA.
Appl Microbiol Biotechnol. 2021 Oct;105(19):7411-7425. doi: 10.1007/s00253-021-11549-8. Epub 2021 Sep 7.
Rhodosporidium toruloides is an oleaginous yeast capable of producing a variety of biofuels and bioproducts from diverse carbon sources. Despite numerous studies showing its promise as a platform microorganism, little is known about its metabolism and physiology. In this work, we investigated the central carbon metabolism in R. toruloides IFO0880 using transcriptomics and metabolomics during growth on glucose, xylose, acetate, or soybean oil. These substrates were chosen because they can be derived from plants. Significant changes in gene expression and metabolite concentrations were observed during growth on these four substrates. We mapped these changes onto the governing metabolic pathways to better understand how R. toruloides reprograms its metabolism to enable growth on these substrates. One notable finding concerns xylose metabolism, where poor expression of xylulokinase induces a bypass leading to arabitol production. Collectively, these results further our understanding of central carbon metabolism in R. toruloides during growth on different substrates. They may also help guide the metabolic engineering and development of better models of metabolism for R. toruloides.Key points• Gene expression and metabolite concentrations were significantly changed.• Reduced expression of xylulokinase induces a bypass leading to arabitol production.• R. toruloides reprograms its metabolism to allow growth on different substrates.
红色红酵母是一种产油酵母,能够利用各种碳源生产多种生物燃料和生物制品。尽管有许多研究表明它有作为平台微生物的潜力,但对其代谢和生理学知之甚少。在这项工作中,我们使用转录组学和代谢组学研究了红色红酵母 IFO0880 在葡萄糖、木糖、乙酸或大豆油上生长时的中心碳代谢。选择这些底物是因为它们可以从植物中获得。在生长过程中观察到这四种底物的基因表达和代谢物浓度有显著变化。我们将这些变化映射到控制代谢途径上,以更好地理解红色红酵母如何重新编程其代谢以适应这些底物的生长。一个值得注意的发现涉及木糖代谢,木糖激酶表达水平低会导致旁路途径,导致阿拉伯糖醇的产生。总的来说,这些结果进一步加深了我们对不同底物生长时红色红酵母中心碳代谢的理解。它们还可能有助于指导红色红酵母代谢工程和更好的代谢模型的开发。关键点:
基因表达和代谢物浓度有显著变化。
木糖激酶表达水平降低会导致旁路途径,导致阿拉伯糖醇的产生。
红色红酵母重新编程其代谢以适应不同的底物生长。