Park Young-Kyoung, Nicaud Jean-Marc
Micalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78352 Jouy-en-Josas, France.
Microorganisms. 2020 Dec 6;8(12):1937. doi: 10.3390/microorganisms8121937.
Using microorganisms as lipid-production factories holds promise as an alternative method for generating petroleum-based chemicals. The non-conventional yeast is an excellent microbial chassis; for example, it can accumulate high levels of lipids and use a broad range of substrates. Furthermore, it is a species for which an array of efficient genetic engineering tools is available. To date, extensive work has been done to metabolically engineer to produce usual and unusual lipids. Unusual lipids are scarce in nature but have several useful applications. As a result, they are increasingly becoming the targets of metabolic engineering. Unusual lipids have distinct structures; they can be generated by engineering endogenous lipid synthesis or by introducing heterologous enzymes to alter the functional groups of fatty acids. In this review, we describe current metabolic engineering strategies for improving lipid production and highlight recent researches on unusual lipid production in .
利用微生物作为脂质生产工厂有望成为生产石油基化学品的替代方法。非常规酵母是一种优秀的微生物底盘；例如，它可以积累高水平的脂质并利用多种底物。此外，它是一种拥有一系列高效基因工程工具的物种。迄今为止，已经开展了大量工作来对[具体酵母名称]进行代谢工程改造以生产常见和不常见的脂质。不常见的脂质在自然界中很稀缺，但有多种有用的应用。因此，它们越来越成为代谢工程的目标。不常见的脂质具有独特的结构；它们可以通过改造内源性脂质合成或引入异源酶来改变脂肪酸的官能团而产生。在本综述中，我们描述了当前用于提高脂质产量的代谢工程策略，并重点介绍了[具体酵母名称]中不常见脂质生产的最新研究。
