Makino S, Kurazono H, Chongsanguam M, Hayashi H, Cheun H, Suzuki S, Shirahata T
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Vet Med Sci. 1999 Nov;61(11):1245-7. doi: 10.1292/jvms.61.1245.
We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.
我们利用沙门氏菌肠毒素基因（stn）建立了针对沙门氏菌属的PCR检测系统。分别使用胰蛋白胨大豆肉汤或沙门氏菌增菌肉汤进行增菌处理后，该检测系统对每克粪便和碎肉样品的检测限为一个细菌细胞。我们得出结论，该PCR系统在公共卫生领域的实际应用中很有用。
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