火棉胶-石蜡夹心切片法：一种制备连续半薄切片的新颖快速方法。

Celloidin-wax sandwich microtomy: a novel and rapid method for producing serial semithin sections.

作者信息

Tyrer N M

机构信息

Department of Optometry and Neuroscience, University of Manchester Institute of Science and Technology, Manchester, M60 1QD, U.K.

出版信息

J Microsc. 1999 Dec;196(Pt 3):273-8. doi: 10.1046/j.1365-2818.1999.00568.x.

DOI:10.1046/j.1365-2818.1999.00568.x

PMID:10594767

Abstract

A method is described that allows rapid and reliable serial sectioning down to thicknesses of 1 micron. The tissue is first embedded in celloidin and then in wax and trimmed so that the block is sandwiched between two layers of wax. This combines the virtues of both media. The celloidin gives greater support to tissue than wax and enables the cutting of semithin sections. The wax allows ribbons of serial sections to be produced as in conventional wax microtomy. This makes it easy to produce serial semithin sections as a matter of routine.

摘要

本文描述了一种方法，该方法能够快速、可靠地进行连续切片，切片厚度可达1微米。首先将组织包埋在火棉胶中，然后再包埋在石蜡中并进行修整，使组织块夹在两层石蜡之间。这结合了两种介质的优点。火棉胶比石蜡对组织的支撑性更好，能够切割半薄切片。石蜡则可以像传统石蜡切片法那样制作连续切片条带。这使得常规制作连续半薄切片变得容易。