Campbell R D
Stain Technol. 1981 Jul;56(4):247-9. doi: 10.3109/10520298109067319.
Epoxy resin sections form strong, heat resistant ribbons if, prior to sectioning, contact cement has been painted onto the leading and trailing faces of the block. The forming ribbon floats onto a drop of water held in place by a wax line drawn across the back of the glass knife parallel to the cutting edge. A long trough made from stainless steel tubing is inserted horizontally into the drop, and as the ribbon lengthens it is directed into the trough. The ribbon can be carried in the trough to a hot plate for expansion and then poured onto a slide for mounting. The serial ribbons obtainable by this simple procedure greatly facilitate three dimensional reconstruction of fine tissue structures.
如果在切片前将接触胶涂在包埋块的前缘和后缘,环氧树脂切片会形成坚固、耐热的条带。形成的条带漂浮在由平行于刀刃在玻璃刀背面绘制的蜡线固定位置的一滴水上。将一个由不锈钢管制成的长槽水平插入水滴中,随着条带变长,它会被引导到槽中。条带可以在槽中被带到热板上进行伸展,然后倒在载玻片上进行固定。通过这个简单程序可获得的连续条带极大地促进了精细组织结构的三维重建。