在基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）过程中，通过源内衰变定位和鉴定翻译后修饰。
Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry.
作者信息
Lennon J J, Walsh K A
机构信息
Department of Biochemistry, University of Washington, Seattle 98195-7350, USA.
出版信息
Protein Sci. 1999 Nov;8(11):2487-93. doi: 10.1110/ps.8.11.2487.
Abstract
A technique is described for identifying and locating posttranslational modifications (PTMs) in peptides and proteins of known sequence by interpretation of c(n) ion signals generated by in-source decay during delayed ion extraction in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sites of phosphorylation in seven synthetic peptides were determined, as was the location of both the heme group and N,N,N-trimethyllysine in yeast cytochrome c. A semi-automated data analysis process facilitates the identification of segments of the sequence on each side of the PTM, permitting its placement at the junction of the segments and definition of the added mass. A graphical display facilitates illustration of both the location and mass of the PTM.
摘要
本文描述了一种技术，通过解释基质辅助激光解吸/电离飞行时间质谱中延迟离子提取期间源内衰变产生的c(n)离子信号，来识别和定位已知序列的肽和蛋白质中的翻译后修饰（PTM）。确定了七种合成肽中的磷酸化位点，以及酵母细胞色素c中血红素基团和N,N,N-三甲基赖氨酸的位置。半自动数据分析过程有助于识别PTM两侧的序列片段，从而将其定位在片段的交界处并确定添加的质量。图形显示便于说明PTM的位置和质量。
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