在延迟离子提取过程中通过源内裂解对蛋白质进行直接序列分析。
Direct sequence analysis of proteins by in-source fragmentation during delayed ion extraction.
作者信息
Lennon J J, Walsh K A
机构信息
Department of Biochemistry, University of Washington, Seattle 98195-7350, USA.
出版信息
Protein Sci. 1997 Nov;6(11):2446-53. doi: 10.1002/pro.5560061118.
Abstract
Continuous segments of amino acid sequence information as long as 41 residues have been deduced by interpretation of matrix-assisted laser desorption/ionization-generated ion signals dominated by Cn fragmentation within the ion source of a linear time-of-flight mass spectrometer utilizing delayed ion extraction. The technique has been applied successively to five proteins of mass 12.2 kDa to 18.3 kDa, yielding segments of continuous sequence as long as 41 residues without the need for prior proteolytic fragmentation. Intact crosslinks such as disulfides or heme linkages interrupt the generation of these data.
摘要
通过对基质辅助激光解吸/电离产生的离子信号进行解析，已经推导得到了长达41个残基的连续氨基酸序列信息。这些离子信号由线性飞行时间质谱仪离子源内的Cn碎裂主导，并采用了延迟离子提取技术。该技术已相继应用于5种质量在12.2 kDa至18.3 kDa之间的蛋白质，无需事先进行蛋白水解断裂，即可得到长达41个残基的连续序列片段。完整的交联结构，如二硫键或血红素键，会干扰这些数据的生成。
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