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Fast isolation of recombinant baculovirus by antibody screening.

作者信息

Grosse F, Manns A

机构信息

Institut für Molekulare Biotechnologie, Abt. Biochemie, Jena, Germany.

出版信息

Mol Biotechnol. 1999 Sep;12(2):217-21. doi: 10.1385/MB:12:2:217.

DOI:10.1385/MB:12:2:217
PMID:10596377
Abstract

We describe a rapid and efficient scheme for the isolation and purification of recombinant baculoviruses. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within 2 to 3 wk. This procedure selects for recombinant baculoviruses that highly overproduce the desired protein product.

摘要

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本文引用的文献

1
Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization.通过有限稀释法和DNA斑点杂交法在杆状病毒表达系统中检测和纯化重组人B淋巴细胞嗜性病毒（HHV-6）
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An efficient procedure for the isolation of recombinant baculovirus.一种高效的重组杆状病毒分离方法。
Nucleic Acids Res. 1989 Jan 11;17(1):451. doi: 10.1093/nar/17.1.451.
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Screening recombinant baculovirus plaques in situ with antibody probes.
Gene Anal Tech. 1989 May-Jun;6(3):62-6. doi: 10.1016/0735-0651(89)90012-5.
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Fast isolation of recombinant baculovirus by antibody screening.通过抗体筛选快速分离重组杆状病毒。
Biotechniques. 1991 Feb;10(2):154-8.