Fung M C, Chiu K Y, Weber T, Chang T W, Chang N T
Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030.
J Virol Methods. 1988 Jan;19(1):33-42. doi: 10.1016/0166-0934(88)90005-5.
A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinant virus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization. This method allows for a quick and convenient way of detection and purification of recombinant virus without the need to use a plaque purification step. It is generally applicable to other expression systems besides the baculovirus system described here.
在杆状病毒表达系统中，通过有限稀释和DNA斑点杂交相结合的方法，从野生型和重组病毒的转染混合物中检测并纯化出重组病毒Ac373-HB。该方法无需进行噬斑纯化步骤，即可快速、便捷地检测和纯化重组病毒。除本文所述的杆状病毒系统外，该方法通常也适用于其他表达系统。
