Sozmen M, Brown P, Eveson J
Department of Pathology and Microbiology, School of Veterinary Science, University of Bristol Veterinary School, Langford, UK.
Vet Res. 1999 Nov-Dec;30(6):583-93.
The distribution of different carbohydrates in dog major and minor salivary glands was investigated using a peroxidase-labelled avidin-biotin method to demonstrate binding of six lectins (Canavalia ensiformis agglutinin [Con A], Dolichos biflorus agglutinin [DBA], Arachis hypogaea (peanut) agglutinin [PNA], Glycine max agglutinin [SBA], Tetragonolobus purpurea agglutinin [TGP] and wheat germ agglutinin [WGA]). With PNA, there was only weak staining in serous acini of parotid glands. Other lectins bound, to different degrees, to different components of the salivary glands; differences could be detected between glands and between binding of different lectins to serous and mucous acinar cells and to the epithelial cell cytoplasm, luminal surface and contents of ducts. These results provide a basis for the comparison of possible changes in carbohydrates which may occur in salivary gland diseases.
采用过氧化物酶标记抗生物素蛋白-生物素方法，研究了六种凝集素（刀豆凝集素[Con A]、双花扁豆凝集素[DBA]、花生凝集素[PNA]、大豆凝集素[SBA]、紫花四棱豆凝集素[TGP]和麦胚凝集素[WGA]）在犬大唾液腺和小唾液腺中不同碳水化合物的分布情况，以显示其结合情况。使用PNA时，腮腺浆液性腺泡仅有微弱染色。其他凝集素与唾液腺的不同成分有不同程度的结合；在不同腺体之间以及不同凝集素与浆液性腺泡细胞和黏液性腺泡细胞、导管上皮细胞质、管腔表面及内容物的结合之间均可检测到差异。这些结果为比较唾液腺疾病中可能出现的碳水化合物变化提供了依据。
