Ishizuya-Oka A, Shimozawa A
Department of Anatomy, School of Medicine, Dokkyo University, Tochigi, Japan.
J Morphol. 1990 Jul;205(1):1-8. doi: 10.1002/jmor.1052050102.
The distribution of structural and secretory glycoconjugates in the gastric region of metamorphosing Xenopus laevis was studied by the avidin-biotin-peroxidase (ABC) histochemical staining method using seven lectins (concanavalin A, Con A; Dolichos biflorus agglutinin, DBA; peanut agglutinin, PNA; Ricinus communis agglutinin I, RCA-I; soybean agglutinin, SBA; Ulex europeus agglutinin I, UEA-I; and wheat germ agglutinin, WGA). Throughout the larval period to stage 60, the epithelium consisting of surface cells and gland cells was stained in various patterns with all lectins examined, whereas the thin layer of connective tissue was positive only for RCA-I. At the beginning of metamorphic climax, the connective tissue became stained with Con A, SBA, and WGA, and its staining pattern varied with different lectins. The region just beneath the surface cells was strongly stained only with RCA-I. With the progression of development, both the epithelium and the connective tissue gradually changed their staining patterns. The surface cells, the gland cells, and the connective tissue conspicuously changed their staining patterns, respectively, for Con A and WGA; for Con A, PNA, RCA-I, SBA, and WGA; and for Con A, RCA-I, and WGA. At the completion of metamorphosis (stage 66), mucous neck cells became clearly identifiable in the epithelium, and their cytoplasm was strongly stained with DBA, PNA, RCA-I, and SBA. These results indicate that lectin histochemistry can provide good criteria for distinguishing among three epithelial cell types, namely, surface cells, gland cells, and mucous neck cells, and between adult and larval cells of each type.
利用七种凝集素（刀豆球蛋白A，Con A；双花扁豆凝集素，DBA；花生凝集素，PNA；蓖麻凝集素I，RCA-I；大豆凝集素，SBA；欧洲荆豆凝集素I，UEA-I；以及麦胚凝集素，WGA），通过抗生物素蛋白-生物素-过氧化物酶（ABC）组织化学染色法，研究了非洲爪蟾变态过程中胃部区域结构和分泌性糖缀合物的分布。在整个幼虫期到第60阶段，由表面细胞和腺细胞组成的上皮细胞用所有检测的凝集素呈现出不同的染色模式，而结缔组织薄层仅对RCA-I呈阳性。在变态高潮开始时，结缔组织开始被Con A、SBA和WGA染色，并且其染色模式因不同凝集素而异。表面细胞正下方的区域仅被RCA-I强烈染色。随着发育的进行，上皮细胞和结缔组织的染色模式逐渐改变。表面细胞、腺细胞和结缔组织分别对Con A和WGA；对Con A、PNA、RCA-I、SBA和WGA；以及对Con A、RCA-I和WGA明显改变了它们的染色模式。在变态完成时（第66阶段），黏液颈细胞在上皮细胞中清晰可辨，并且它们的细胞质被DBA、PNA、RCA-I和SBA强烈染色。这些结果表明，凝集素组织化学可以为区分三种上皮细胞类型，即表面细胞、腺细胞和黏液颈细胞，以及每种类型的成体细胞和幼虫细胞提供良好的标准。
