Lutsik A D, Iashchenko A M, Detiuk E S, Lutsik M D
Arkh Anat Gistol Embriol. 1986 Aug;91(8):27-35.
Distribution of lectin-binding sites in rat submandibular and sublingual salivary glands during postnatal development has been investigated. Lectin preparations include con A, lentin lectin, castor beans agglutinin, peanut, soybean and Sophora japonica agglutinins, wheat germ agglutinin and lectin from the bark of Laburnum anagyroides. The direct and indirect peroxidase techniques are used. According to the similarities of histochemical patterns, all lectins are divided into four groups. Besides the general patterns of lectin binding sites, some details are noted. Lectins of peanut and Sophora japonica possess an extremely high affinity to mast cells, con A, lens lectin, castor beans and wheat germ agglutinins--to serous demilunes cells. Laburnum lectin--to salivary ducts epithelia in adult rat salivary glands. Lentin lectin, con A and Laburnum lectin preferentially stain cells with specific granularity in granular ducts at early stages of postnatal development. Considering the character of staining, we propose for further histochemical investigations of the salivary glands lentin lectin, peanut agglutinin, wheat germ agglutinin and Laburum anagyroides lectin.
对出生后发育过程中大鼠下颌下腺和舌下腺中凝集素结合位点的分布进行了研究。凝集素制剂包括刀豆球蛋白A、香菇凝集素、蓖麻凝集素、花生凝集素、大豆凝集素、槐凝集素、小麦胚凝集素以及金链花树皮凝集素。采用直接和间接过氧化物酶技术。根据组织化学模式的相似性，所有凝集素被分为四组。除了凝集素结合位点的一般模式外，还注意到一些细节。花生凝集素和槐凝集素对肥大细胞具有极高的亲和力，刀豆球蛋白A、扁豆凝集素、蓖麻凝集素和小麦胚凝集素对浆液半月细胞具有亲和力。金链花凝集素对成年大鼠唾液腺的唾液导管上皮具有亲和力。香菇凝集素、刀豆球蛋白A和金链花凝集素在出生后发育早期优先对颗粒导管中具有特定颗粒度的细胞进行染色。考虑到染色特性，我们建议对唾液腺进行进一步的组织化学研究时使用香菇凝集素、花生凝集素、小麦胚凝集素和金链花凝集素。
