Short S C, Mitchell S A, Boulton P, Woodcock M, Joiner M C
Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK.
Int J Radiat Biol. 1999 Nov;75(11):1341-8. doi: 10.1080/095530099139214.
To examine the low-dose radiation response of a series of radioresistant human glioma cell lines and determine if low-dose hypersensitivity is a characteristic of these cells.
The clonogenic survival of six radioresistant human glioma cell lines was measured following exposure to graded, single, very low doses of X-rays in vitro. High resolution was achieved using either a Dynamic Microscopic Image Processing Scanner (DMIPS) or a cell sorter (CS).
In five of the six cell lines tested, low-dose hypersensitivity (HRS) was demonstrated although in the sixth, a grade III astrocytoma line, it was not. These results are consistent with previous data indicating that low-dose hypersensitivity is more marked in more radioresistant cell lines although the difference between the glioblastoma cell lines with differing SF2 is not marked.
Low-dose hypersensitivity is common in radioresistant glioma cell lines. This may have implications for the treatment of these tumours if further studies confirm that HRS translates to increased effectiveness per gray in vivo when very low doses per fraction are used.
检测一系列耐辐射的人胶质瘤细胞系的低剂量辐射反应，并确定低剂量超敏反应是否为这些细胞的特征。
在体外对六种耐辐射的人胶质瘤细胞系进行分级、单次、极低剂量X射线照射后，测量其克隆形成存活率。使用动态显微镜图像处理扫描仪（DMIPS）或细胞分选仪（CS）实现高分辨率。
在所测试的六种细胞系中的五种中表现出低剂量超敏反应（HRS），尽管在第六种细胞系（III级星形细胞瘤细胞系）中未表现出。这些结果与先前的数据一致，表明低剂量超敏反应在更耐辐射的细胞系中更为明显，尽管具有不同SF2的胶质母细胞瘤细胞系之间的差异并不显著。
低剂量超敏反应在耐辐射的胶质瘤细胞系中很常见。如果进一步的研究证实当使用极低的分次剂量时HRS在体内每格雷能转化为更高的有效性，那么这可能对这些肿瘤的治疗具有意义。
