Monti J, Fay M, Banchio C, Améndola R, Farías R, Musi A, Sánchez A, Kesner L, Katz J, Ferro F, Kogan Z, Corti R
Hospital de Gastroenterología Dr. Carlos Bonorino Udaondo Buenos Aires, Argentina.
Acta Gastroenterol Latinoam. 1999;29(4):251-3.
Some papers report helicobacter pylori existence in bile from surgical specimens obtained during gallbladder or bile ducts surgery. The aim of this work was search by PCR, H. Pylori presence in bile specimens from patients suffering of gallbladder stones or by bile ducts stones. Bile samples were obtained by gallbladder punction during cholecystectomy in 26 patients, 19 of them with gallbladder stones and 7 also with gallbladder stones and bile duct stones. Age ranged from 22-69 years old, median 49.6 years old. Samples were sent to specialized biomolecular laboratory to perform PCR techniques. Two of 26 patients (7.6%) had positive reaction for the presence of DNA of H. Pylori in bile samples. Our research suggest that DNA of H. Pylori can be founded in bile samples patients with gallbladders and duct stones in Argentina.
一些论文报道在胆囊或胆管手术中获取的手术标本胆汁中存在幽门螺杆菌。本研究的目的是通过聚合酶链反应（PCR）检测胆囊结石或胆管结石患者胆汁标本中幽门螺杆菌的存在情况。在26例患者行胆囊切除术时通过胆囊穿刺获取胆汁样本，其中19例有胆囊结石，7例既有胆囊结石又有胆管结石。年龄范围为22至69岁，中位数为49.6岁。样本被送至专业生物分子实验室进行PCR技术检测。26例患者中有2例（7.6%）胆汁样本中幽门螺杆菌DNA检测呈阳性反应。我们的研究表明，在阿根廷，胆囊和胆管结石患者的胆汁样本中可检测到幽门螺杆菌DNA。
