Dar Mohammad Yousuf, Ali Sadaf, Raina Abdul Hameed, Raina Manzoor A, Shah O J, Shah Mubashir A, Mudassar Syed
Department of General and Minimal Invasive Surgery, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India.
Department of Surgical Gastroenterology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India.
Indian J Gastroenterol. 2016 Sep;35(5):343-346. doi: 10.1007/s12664-016-0675-7. Epub 2016 Sep 16.
Hepatobiliary stone disease is one of the most common surgical conditions worldwide. There are multiple causative agents responsible for the formation of hepatobiliary stones, and bacterial infection is one of them. The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confounding factors, or ethical and regional differences among populations.
We investigated the presence of Helicobacter pylori species by PCR of 26-kDa surface antigen specific to H. pylori in bile samples from 50 cases with hepatobiliary stones and 25 controls without hepatobiliary stones. The control group comprised of 20 patients of hydatid cyst disease of liver and 5 patients of right colonic growth.
H. pylori was present in 20 bile samples among cases and was absent in 30 bile samples among cases. Among controls, H. pylori could not be detected. A significant association of the presence of H. pylori with hepatobiliary stone disease was seen (p < 0.001).
There is an association between bile infection with H. pylori and hepatobiliary stone disease.
肝胆结石病是全球最常见的外科疾病之一。导致肝胆结石形成的病原体有多种，细菌感染是其中之一。人们已经在胆道疾病患者的胆管上皮中研究了幽门螺杆菌DNA种类的存在情况。然而，观察到的结果相互矛盾，这可能是由于研究对象数量少、难以获得健康对照组、未控制混杂因素或人群之间的伦理和地区差异所致。
我们通过对50例肝胆结石患者和25例无肝胆结石的对照者胆汁样本中幽门螺杆菌特异性26 kDa表面抗原进行聚合酶链反应（PCR），来研究幽门螺杆菌种类的存在情况。对照组包括20例肝包虫囊肿病患者和5例右半结肠肿物患者。
病例组中有20份胆汁样本检测到幽门螺杆菌，30份胆汁样本未检测到。对照组中未检测到幽门螺杆菌。幽门螺杆菌的存在与肝胆结石病之间存在显著关联（p < 0.001）。
幽门螺杆菌感染胆汁与肝胆结石病之间存在关联。
