Frank U, Rinkevich B
The National Institute of Oceanography, Israel Oceanographic and Limnological Research, Haifa, 31080, Israel.
Cell Biol Int. 1999;23(4):307-11. doi: 10.1006/cbir.1998.0352.
Mechanically and enzymatically dissociated cells from five anthozoan species were laid on seven substrates in vitro. Cells were taken from two sea anemones (Aiptasia sp. and Anemonia sulcata), a scleractinian coral (Stylophora pistillata) and two alcyonacean corals (Heteroxenia fuscescence and Nephthea sp). Substrates tested: glass (coverslips), plastic (uncoated tissue culture plates), type IV collagen, gelatin, fibronectin, mesoglea pieces from the scyphozoan jellyfish Rhopilema nomadica and acetic acid extract of jellyfish mesoglea. Except for the mesoglea pieces, cells did not respond to any one of the other substrates, retaining their rounded shape. Following contact with mesoglea pieces, cells attached and spread. Subsequently they migrated into the mesogleal matrix at a rate of 5-10 microm/h during the first 2-5 h. No difference was found between the behavior of cells from the five different cnidarian species.
将来自5种珊瑚虫纲动物的细胞通过机械和酶解法进行解离，然后在体外置于7种基质上。细胞取自两种海葵（艾氏海葵和沟迎风海葵）、一种石珊瑚（鹿角杯形珊瑚）以及两种软珊瑚（褐软珊瑚和肾形真叶软珊瑚）。测试的基质有：玻璃（盖玻片）、塑料（未包被的组织培养板）、IV型胶原、明胶、纤连蛋白、来自硝水母的中胶层碎片以及水母中胶层的乙酸提取物。除了中胶层碎片外，细胞对其他任何一种基质都没有反应，仍保持圆形。与中胶层碎片接触后，细胞附着并铺展。随后，它们在最初的2 - 5小时内以5 - 10微米/小时的速度迁移到中胶层基质中。来自5种不同刺胞动物的细胞行为之间未发现差异。
