Schmid V, Bally A
Institute of Zoology, University of Basle, Switzerland.
Dev Biol. 1988 Oct;129(2):573-81. doi: 10.1016/0012-1606(88)90402-2.
A new system is described for the study of ECM-tissue interactions, using the ECM (called mesogloea) of various cnidarians and isolated striated muscle and endodermal tissue of jellyfish. The mesogloea consists mainly of water and collagen. It is present in all cnidarians and can be isolated without enzyme treatment. It can be used as a substrate to which cells and tissues adhere and on which they spread and migrate. Tissues of striated muscle and endoderm adhere and spread not only on mesogloea from regions they normally cover, but also from other regions of the animal. However, adhesion and spreading are highly species-specific. Species-specific adhesion is found throughout the whole mass of mesogloea even at regions where cells do not occur naturally. The cell adhesion factor can be extracted from the mesogloea so that the mesogloea no longer shows any cell adhesion properties. The extract consists mainly of a cysteine-containing collagen.
本文描述了一种用于研究细胞外基质（ECM）与组织相互作用的新系统，该系统使用各种刺胞动物的ECM（称为中胶层）以及水母的分离横纹肌和内胚层组织。中胶层主要由水和胶原蛋白组成。它存在于所有刺胞动物中，无需酶处理即可分离。它可以用作细胞和组织粘附、铺展和迁移的底物。横纹肌和内胚层组织不仅粘附并铺展在它们通常覆盖区域的中胶层上，也粘附并铺展在动物其他区域的中胶层上。然而，粘附和铺展具有高度的物种特异性。即使在细胞自然不存在的区域，整个中胶层中都存在物种特异性粘附。细胞粘附因子可以从中胶层中提取出来，这样中胶层就不再显示任何细胞粘附特性。提取物主要由一种含半胱氨酸的胶原蛋白组成。
