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[海月水母中的差异基因表达]

[Differential gene expression in the jellyfish Aurelia aurita].

作者信息

Matveev I V

出版信息

Tsitologiia. 2005;47(5):431-5.

Abstract

The body of Aurelia aurita, as well as other diploblasts, consists of two epithelial layers: ectodermal and gastral epithelium. These two tissues are separated by mesoglea, or extracellular matrix. In most coelenterates mesoglea is acellular. In A. aurita mesogleal cells are scattered in mesoglea. Differential display PCR was used to compare mRNA pools from ectodermal epithelium, gastral epithelium and mesoglea. 4 novel gene fragments were cloned and sequenced. According to RTPCR results, one of these fragments is differentially expressed in the ectodermal epithelium.

摘要

海月水母以及其他双胚层动物的身体由两个上皮层组成

外胚层上皮和胃上皮。这两种组织被中胶层或细胞外基质分隔开。在大多数腔肠动物中,中胶层是无细胞的。在海月水母中,中胶层细胞散布在中胶层中。采用差异显示PCR技术比较外胚层上皮、胃上皮和中胶层的mRNA库。克隆并测序了4个新的基因片段。根据逆转录PCR结果,其中一个片段在外胚层上皮中差异表达。

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