Ishine N, Rubinsky B, Lee C Y
Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.
Cryobiology. 1999 Nov;39(3):271-7. doi: 10.1006/cryo.1999.2205.
As part of a more extensive study on the use of high subzero freezing for cryopreservation of mammalian livers we have tried to single out the effects of freezing and thawing on tissue damage. We compared the morphology of livers after freezing and thawing with what we considered an optimal high subzero cryopreservation protocol with the morphology of livers preserved under the same thermal conditions and in the same solution in a supercooled state, without freezing. The results show that while hepatocytes survive high subzero cryopreservation, detachment of endothelial cells occurs in every freezing experiment. On the other hand, the endothelial cells in livers that are not frozen are intact. This suggests that endothelial cell damage is caused by freezing and may be an important factor in high subzero freezing cryopreservation of the liver.
作为关于使用零下高温度冷冻法保存哺乳动物肝脏的更广泛研究的一部分，我们试图找出冷冻和解冻对组织损伤的影响。我们将冷冻和解冻后的肝脏形态与我们认为的最佳零下高温度冷冻保存方案下的肝脏形态进行了比较，同时也将其与在相同热条件下、处于过冷状态（未冷冻）且在相同溶液中保存的肝脏形态进行了比较。结果表明，虽然肝细胞在零下高温度冷冻保存中能够存活，但在每次冷冻实验中都会出现内皮细胞脱离的情况。另一方面，未冷冻肝脏中的内皮细胞是完整的。这表明内皮细胞损伤是由冷冻引起的，并且可能是肝脏零下高温度冷冻保存中的一个重要因素。
