Bischof J C, Rubinsky B
Department of Mechanical Engineering, University of California at Berkeley 94707.
Cryobiology. 1993 Dec;30(6):597-603. doi: 10.1006/cryo.1993.1062.
Evidence in the literature shows that ice crystals that form in the nucleus of many rapidly cooled cells appear much larger than the ice crystals that form in the surrounding cytoplasm. We investigated the phenomenon in our laboratory using the techniques of freeze substitution and low temperature scanning electron microscopy on liver tissue frozen by liquid nitrogen plunge freezing. This method is estimated to cool the tissue at 1000 degrees C/min. The results from these techniques show that the ice crystal sizes were statistically significantly larger in the nucleus than in the cytoplasm. It is our belief that this finding is important to cryobiology considering its potential role in the process of freezing and the mechanisms of damage during freezing of cells and tissues.
文献中的证据表明，在许多快速冷却细胞的细胞核中形成的冰晶，看起来比在周围细胞质中形成的冰晶大得多。我们在实验室中使用冷冻置换和低温扫描电子显微镜技术，对通过液氮骤冷冷冻的肝脏组织进行了研究。据估计，这种方法能使组织以每分钟1000摄氏度的速度冷却。这些技术的结果表明，细胞核中的冰晶尺寸在统计学上显著大于细胞质中的冰晶尺寸。我们认为，这一发现对低温生物学很重要，因为它在细胞和组织冷冻过程及冷冻损伤机制中可能发挥作用。
