Conte D, Romanelli F, Fillo S, Guidetti L, Isidori A, Franceschi F, Latini M, di Luigi L
Division of Andrology, Department of Medical Pathophysiology, University "La Sapienza," 00161 Rome, Italy.
Am J Physiol. 1999 Dec;277(6):E1032-7. doi: 10.1152/ajpendo.1999.277.6.E1032.
Eicosanoids play an important role in the regulation of the hypothalamic-pituitary axis; less clear is their role in testicular steroidogenesis. To evaluate the involvement of cyclooxygenase metabolites, such as prostaglandins, in the regulation of human testicular steroidogenesis, we examined the effects of a prostaglandin-blocker, aspirin, on plasma testosterone, pregnenolone, progesterone, 17OH-progesterone, androstenedione, dehydroepiandrosterone, and 17beta-estradiol response to human chorionic gonadotropin (hCG) in normal male volunteers in a placebo-controlled, single-blinded study. To test the efficacy of aspirin, seminal prostaglandin E(2) levels were also determined. hCG stimulation increased peripheral levels of testosterone, 17OH-progesterone, androstenedione, dehydroepiandrosterone, and 17beta-estradiol, without affecting circulating pregnenolone and progesterone values. Aspirin significantly lowered seminal prostaglandin E(2) levels, whereas it did not modify steroid concentrations not exposed to exogenous hCG. Moreover, the drug significantly reduced the response of testosterone, 17OH-progesterone, androstenedione, and dehydroepiandrosterone to hCG, as assessed by the mean integrated area under the curve, whereas it did not influence 17beta-estradiol response. In conclusion, aspirin treatment inhibits androgen response to chorionic gonadotropin stimulation in normal humans. The action of aspirin is probably mediated via an effective arachidonate cyclooxygenase block.
类二十烷酸在调节下丘脑 - 垂体轴中发挥重要作用；但其在睾丸类固醇生成中的作用尚不清楚。为了评估环氧化酶代谢产物（如前列腺素）在人类睾丸类固醇生成调节中的作用，我们在一项安慰剂对照、单盲研究中，检测了前列腺素阻滞剂阿司匹林对正常男性志愿者血浆睾酮、孕烯醇酮、孕酮、17α-羟孕酮、雄烯二酮、脱氢表雄酮和17β-雌二醇对人绒毛膜促性腺激素（hCG）反应的影响。为了测试阿司匹林的疗效，还测定了精液中前列腺素E2的水平。hCG刺激可提高外周血睾酮、17α-羟孕酮、雄烯二酮、脱氢表雄酮和17β-雌二醇的水平，而不影响循环中的孕烯醇酮和孕酮值。阿司匹林显著降低了精液中前列腺素E2的水平，而对未暴露于外源性hCG的类固醇浓度没有影响。此外，通过曲线下平均积分面积评估，该药物显著降低了睾酮、17α-羟孕酮、雄烯二酮和脱氢表雄酮对hCG的反应，而不影响17β-雌二醇的反应。总之，阿司匹林治疗可抑制正常人体内雄激素对绒毛膜促性腺激素刺激的反应。阿司匹林的作用可能是通过有效阻断花生四烯酸环氧化酶介导的。
