Aalinkeel R, Srinivasan M, Kalhan S C, Laychock S G, Patel M S
Departments of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Am J Physiol. 1999 Dec;277(6):E1061-9. doi: 10.1152/ajpendo.1999.277.6.E1061.
Artificial rearing of 4-day-old rat pups on a high-carbohydrate (HC) milk formula results in the immediate onset of hyperinsulinemia. To evaluate these early changes, studies on pancreatic function were carried out on 12-day-old HC rats and compared with age-matched mother-fed (MF) pups. The plasma insulin and glucagon contents were increased sixfold and twofold, respectively, in HC rats compared with MF rats. There was a distinct leftward shift in the glucose-stimulated insulin secretory pattern for HC islets. HC islets secreted insulin in the absence of any added glucose and in the presence of Ca(2+) channel inhibitors. The activities of glucokinase, hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate dehydrogenase complex were significantly increased in HC islets compared with MF islets. The protein contents of GLUT-2 and hexokinase were significantly increased in HC islets. These findings indicate that a nutritional intervention in the form of a HC formula only during the suckling period has a profound influence on pancreatic function, causing the onset of hyperinsulinemia.
用高碳水化合物（HC）牛奶配方人工饲养4日龄大鼠幼崽会导致立即出现高胰岛素血症。为了评估这些早期变化，对12日龄的HC大鼠进行了胰腺功能研究，并与年龄匹配的由母鼠喂养（MF）的幼崽进行比较。与MF大鼠相比，HC大鼠的血浆胰岛素和胰高血糖素含量分别增加了6倍和2倍。HC胰岛的葡萄糖刺激胰岛素分泌模式明显向左移位。HC胰岛在未添加任何葡萄糖以及存在Ca(2+)通道抑制剂的情况下分泌胰岛素。与MF胰岛相比，HC胰岛中葡萄糖激酶、己糖激酶、甘油醛-3-磷酸脱氢酶和丙酮酸脱氢酶复合物的活性显著增加。HC胰岛中GLUT-2和己糖激酶的蛋白质含量显著增加。这些发现表明，仅在哺乳期以HC配方形式进行的营养干预对胰腺功能有深远影响，导致高胰岛素血症的发生。
