Liu Jia, Gao Fengyi, Ji Baoping, Wang Ruojun, Yang Junsi, Liu Huijun, Zhou Feng
Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.
Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China ; China Agricultural University, Mailbox #294, #17 Qinghua East Road, Haidian District, Beijing, 100083 People's Republic of China.
J Food Sci Technol. 2015 May;52(5):3022-9. doi: 10.1007/s13197-014-1379-6. Epub 2014 Apr 26.
As commonly observed events in diabetic patients, glucolipotoxicity induces oxidative stress, apoptosis and functional defects in β-cells. Anthocyanins are well investigated as strong antioxidants and modulators for metabolic syndromes. Therefore, this study examined the protective effects of anthocyanins-rich extracts (BAE) from wild Chinese blueberry (Vaccinium spp.) against glucolipotoxicity in β-cells. Results showed that INS832/13 β-cells subjected to glucolipotoxicity were significantly decreased (p < 0.05) in cell survival rate, which were alleviated by BAE and metformin treatments. Both BAE and metformin reduced reactive oxidative species and improved the antioxidant defense system. Moreover, BAE were effective in reducing intracellular triglycerides (TG) level, restoring intracellular insulin content, lowering basal insulin secretion (BIS) and increasing glucose-stimulated insulin secretion which in turn resulted in an elevated insulin secretion index. However, metformin only demonstrated marginal effect on secretion dysfunction and had no effect (p > 0.05) on BIS or TG. Additionally, TG levels reduced by BAE treatment were correlated with BIS (p < 0.01, r = 0.9755). This study has for the first time demonstrated that anthocyanin enriched extract of wild Chinese blueberry could effectively protect β-cells against glucolipotoxicity in vitro. These results implied the potential efficacy of BAE as a complementary measure for diabetes intervention.
作为糖尿病患者中常见的现象，糖脂毒性会诱导β细胞产生氧化应激、细胞凋亡和功能缺陷。花色苷作为强大的抗氧化剂和代谢综合征调节剂已得到充分研究。因此，本研究考察了野生中国蓝莓（越橘属）中富含花色苷的提取物（BAE）对β细胞糖脂毒性的保护作用。结果显示，遭受糖脂毒性的INS832/13β细胞存活率显著降低（p<0.05），而BAE和二甲双胍处理可缓解这一情况。BAE和二甲双胍均能降低活性氧水平并改善抗氧化防御系统。此外，BAE能有效降低细胞内甘油三酯（TG）水平，恢复细胞内胰岛素含量，降低基础胰岛素分泌（BIS）并增加葡萄糖刺激的胰岛素分泌，进而提高胰岛素分泌指数。然而，二甲双胍仅对分泌功能障碍有轻微作用，对BIS或TG无影响（p>0.05）。此外，BAE处理降低的TG水平与BIS相关（p<0.01，r=0.9755）。本研究首次证明，野生中国蓝莓富含花色苷的提取物在体外可有效保护β细胞免受糖脂毒性影响。这些结果表明BAE作为糖尿病干预补充措施的潜在功效。
