Eslava A P, Alvarez M I, Delbrück M
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4076-80. doi: 10.1073/pnas.72.10.4076.
A four-factor cross between two strains of Phycomyces involving two auxotrophic, one color, and the mating type marker is described. Samples of 40 germspores from 84 individual fertile germsporangia were characterized. The results show: (i) The germspores of a germsporangium are derived from one meiosis in approximately 78% of the cases. (ii) The four markers are on separate chromosomes. They are nonselective. (iii) Analysis of a large sample of germspores from 106 pooled germsporangia confirms that the four markers are unlinked. (iv) From the ditype/tetratype ratios it is inferred that each marker is located about 15 map units from its centromere.
描述了两种毛霉属菌株之间涉及两个营养缺陷型、一个颜色和交配型标记的四因子杂交。对来自84个可育孢子囊个体的40个芽孢样本进行了表征。结果表明:(i)在大约78%的情况下,一个孢子囊的芽孢来自一次减数分裂。(ii)这四个标记位于不同的染色体上。它们是非选择性的。(iii)对来自106个合并孢子囊的大量芽孢样本的分析证实,这四个标记是不连锁的。(iv)从双型/四型比率推断,每个标记与其着丝粒的距离约为15个图距单位。