布氏毛霉嘧啶营养缺陷型的分离、特性鉴定及定位
Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.
作者信息
Campuzano V, del Valle P, de Vicente J I, Eslava A P, Alvarez M I
机构信息
Departamento de Microbiología y Genética, Facultad de Biología, Universidad de Salamanca, Spain.
出版信息
Curr Genet. 1993 Dec;24(6):515-9. doi: 10.1007/BF00351715.
A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.
从5-氟乳清酸（5-FOA）抗性突变体中总共分离出7株毛霉嘧啶营养缺陷型菌株。通过互补分析将它们分为两组。属于一组的代表性突变菌株缺乏乳清酸磷酸核糖转移酶（OPRTase；EC 2.4.2.10）活性；属于第二组的突变菌株缺乏乳清苷-5'-单磷酸脱羧酶（OMPdecase；EC 4.1.1.23）。这些突变体分别在pyrF和pyrG基因中存在缺陷。随机孢子分析、四分体分析和基因-着丝粒距离的结果表明，这两个标记位于连锁群VI中，pyrG是近端标记，pyrF是远端标记。
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