Liu Q, Suzuki K, Kudo S, Yamada M, Kowatari K, Umeda T, Nakaji S, Sugawara K
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Luminescence. 1999 Nov-Dec;14(6):327-30. doi: 10.1002/(SICI)1522-7243(199911/12)14:6<327::AID-BIO571>3.0.CO;2-A.
Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.
癸甘油单油酸酯（DGMO）是一种食品添加剂（脂肪酸聚甘油酯），采用鲁米诺依赖性化学发光分析法评估了其对人中性粒细胞产生活性氧（ROS）的体外作用。通过Histopaque-1077/1119双密度梯度技术分离中性粒细胞。调理酵母聚糖（OZ）和佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）用作人工刺激物。在浓度为0.5 mg/mL时，DGMO可使OZ刺激的中性粒细胞的化学发光反应抑制58%（p<0.01）。在浓度为0.01 mg/mL时，DGMO可使PMA诱导的化学发光抑制40%（p<0.01）。抑制现象不能仅归因于DGMO的光吸收，因为存在DGMO时的化学发光百分比低于DGMO在425 nm处的透光率百分比。这些结果表明，DGMO可下调人中性粒细胞的ROS生成。
