肝螺杆菌这一肠肝病原体中的细胞致死膨胀毒素序列及活性
Cytolethal distending toxin sequence and activity in the enterohepatic pathogen Helicobacter hepaticus.
作者信息
Young V B, Knox K A, Schauer D B
机构信息
Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Infect Immun. 2000 Jan;68(1):184-91. doi: 10.1128/IAI.68.1.184-191.2000.
Abstract
Little is known about the molecular pathogenesis of hepatitis and enterocolitis caused by enterohepatic Helicobacter species. Sonicates of the murine pathogen Helicobacter hepaticus were found to cause progressive cell distension, accumulation of filamentous actin, and G(2)/M cell cycle arrest in HeLa cell monolayers. The genes encoding this cytotoxic activity were cloned from H. hepaticus. Three open reading frames with closest homology to cdtA, cdtB, and cdtC from Campylobacter jejuni were identified. Sonicates of a laboratory strain of Escherichia coli carrying the cloned cdtABC gene cluster from H. hepaticus reproduced the cytotoxic activities seen with sonicates of H. hepaticus. Cytolethal distending toxin activity is a potential virulence determinant of H. hepaticus that may play a role in the pathogenesis of Helicobacter-associated hepatitis and enterocolitis.
摘要
关于肝肠螺杆菌引起的肝炎和小肠结肠炎的分子发病机制，人们了解甚少。已发现鼠病原体肝螺杆菌的超声裂解物可导致HeLa细胞单层出现渐进性细胞膨胀、丝状肌动蛋白积累以及G(2)/M期细胞周期阻滞。编码这种细胞毒性活性的基因从肝螺杆菌中克隆出来。鉴定出了三个与空肠弯曲菌的cdtA、cdtB和cdtC具有最高同源性的开放阅读框。携带从肝螺杆菌克隆的cdtABC基因簇的实验室大肠杆菌菌株的超声裂解物重现了肝螺杆菌超声裂解物所具有的细胞毒性活性。细胞致死膨胀毒素活性是肝螺杆菌的一种潜在毒力决定因素，可能在螺杆菌相关肝炎和小肠结肠炎的发病机制中起作用。
相似文献
1
Cytolethal distending toxin sequence and activity in the enterohepatic pathogen Helicobacter hepaticus.
Infect Immun. 2000 Jan;68(1):184-91. doi: 10.1128/IAI.68.1.184-191.2000.
2
Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.
J Med Microbiol. 2000 Jun;49(6):525-534. doi: 10.1099/0022-1317-49-6-525.
3
Comparative analysis of cytolethal distending toxin (cdt) genes among Campylobacter jejuni, C. coli and C. fetus strains.
Microb Pathog. 2007 May-Jun;42(5-6):174-83. doi: 10.1016/j.micpath.2007.01.005. Epub 2007 Feb 6.
4
Cytolethal distending toxin: a potential virulence factor for Helicobacter cinaedi.
J Infect Dis. 2003 Dec 15;188(12):1892-7. doi: 10.1086/379837. Epub 2003 Dec 9.
5
In vitro and in vivo characterization of Helicobacter hepaticus cytolethal distending toxin mutants.
Infect Immun. 2004 May;72(5):2521-7. doi: 10.1128/IAI.72.5.2521-2527.2004.
6
Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira.
J Med Microbiol. 2003 Feb;52(Pt 2):103-108. doi: 10.1099/jmm.0.04920-0.
7
Interactions of Campylobacter jejuni cytolethal distending toxin subunits CdtA and CdtC with HeLa cells.
Infect Immun. 2003 Sep;71(9):4883-90. doi: 10.1128/IAI.71.9.4883-4890.2003.
8
Contribution of Helicobacter hepaticus cytolethal distending toxin subunits to human epithelial cell cycle arrest and apoptotic death in vitro.
Helicobacter. 2013 Dec;18(6):433-43. doi: 10.1111/hel.12084. Epub 2013 Jul 29.
9
A new variant of cytolethal distending toxin in a clinical isolate of Campylobacter hyointestinalis.
J Med Microbiol. 2015 Oct;64(10):1124-1134. doi: 10.1099/jmm.0.000145. Epub 2015 Jul 27.
10
Expression and activity of the cytolethal distending toxin of Helicobacter hepaticus.
Biochem Biophys Res Commun. 2004 Jun 4;318(3):739-45. doi: 10.1016/j.bbrc.2004.04.089.
引用本文的文献
1
Pathogenicity of Arcobacter cryaerophilus in two human intestinal cell lines.
Gut Pathog. 2025 Jun 22;17(1):48. doi: 10.1186/s13099-025-00721-4.
2
Chicken as a carrier of emerging virulent Helicobacter species: a potential zoonotic risk.
Gut Pathog. 2025 May 21;17(1):31. doi: 10.1186/s13099-025-00707-2.
3
Cytolethal distending toxin: from genotoxin to a potential biomarker and anti-tumor target.
World J Microbiol Biotechnol. 2021 Aug 11;37(9):150. doi: 10.1007/s11274-021-03117-z.
4
Intestinal Inflammation and Altered Gut Microbiota Associated with Inflammatory Bowel Disease Render Mice Susceptible to Clostridioides difficile Colonization and Infection.
mBio. 2021 Jun 29;12(3):e0273320. doi: 10.1128/mBio.02733-20. Epub 2021 Jun 15.
5
The Majority of Typhoid Toxin-Positive Serovars Encode ArtB, an Alternate Binding Subunit.
mSphere. 2021 Jan 6;6(1):e01255-20. doi: 10.1128/mSphere.01255-20.
6
Complete Genome Sequencing and Comparative Genomic Analysis of Isolated From the Wild Korean Striped Field Mouse ) for Potential Pathogenicity.
Front Pharmacol. 2018 Jul 31;9:838. doi: 10.3389/fphar.2018.00838. eCollection 2018.
7
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia.
J Vet Med Sci. 2018 Feb 2;80(1):138-146. doi: 10.1292/jvms.17-0492. Epub 2017 Dec 15.
8
CdtC-Induced Processing of Membrane-Bound CdtA Is a Crucial Step in Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Holotoxin Formation.
Infect Immun. 2018 Feb 20;86(3). doi: 10.1128/IAI.00731-17. Print 2018 Mar.
9
The Cytolethal Distending Toxin Subunit CdtB of Promotes Senescence and Endoreplication in Xenograft Mouse Models of Hepatic and Intestinal Cell Lines.
Front Cell Infect Microbiol. 2017 Jun 30;7:268. doi: 10.3389/fcimb.2017.00268. eCollection 2017.
10
Haemophilus parasuis cytolethal distending toxin induces cell cycle arrest and p53-dependent apoptosis.
PLoS One. 2017 May 18;12(5):e0177199. doi: 10.1371/journal.pone.0177199. eCollection 2017.
本文引用的文献
1
Characterization of a Haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin.
Infect Immun. 1999 Aug;67(8):3900-8. doi: 10.1128/IAI.67.8.3900-3908.1999.
2
Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR.
J Clin Microbiol. 1999 May;37(5):1646-50. doi: 10.1128/JCM.37.5.1646-1650.1999.
3
Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.
Nature. 1999 Jan 14;397(6715):176-80. doi: 10.1038/16495.
4
IL-7 deficiency prevents development of a non-T cell non-B cell-mediated colitis.
J Immunol. 1998 Nov 15;161(10):5673-80.
5
Impact of Helicobacter hepaticus infection in B6C3F1 mice from twelve National Toxicology Program two-year carcinogenesis studies.
Toxicol Pathol. 1998 Sep-Oct;26(5):602-11. doi: 10.1177/019262339802600503.
6
Helicobacter hepaticus triggers colitis in specific-pathogen-free interleukin-10 (IL-10)-deficient mice through an IL-12- and gamma interferon-dependent mechanism.
Infect Immun. 1998 Nov;66(11):5157-66. doi: 10.1128/IAI.66.11.5157-5166.1998.
7
The cell cycle-specific growth-inhibitory factor produced by Actinobacillus actinomycetemcomitans is a cytolethal distending toxin.
Infect Immun. 1998 Oct;66(10):5008-19. doi: 10.1128/IAI.66.10.5008-5019.1998.
8
Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum.
J Appl Microbiol. 1998 Jun;84(6):1017-24. doi: 10.1046/j.1365-2672.1998.00438.x.
9
Chronic active hepatitis induced by Helicobacter hepaticus in the A/JCr mouse is associated with a Th1 cell-mediated immune response.
Infect Immun. 1998 Jul;66(7):3142-8. doi: 10.1128/IAI.66.7.3142-3148.1998.
10
Analyses of the cag pathogenicity island of Helicobacter pylori.
Mol Microbiol. 1998 Apr;28(1):37-53. doi: 10.1046/j.1365-2958.1998.00770.x.
Zotero 插件