Bachus Antonia, Beyer Sarah, Bücker Roland, Sharbati Soroush, Alter Thomas, Gölz Greta
Institute of Food Safety and Food Hygiene, Freie Universität Berlin, Berlin, Germany.
Clinical Physiology/Nutritional Medicine, Charité- Universitätsmedizin Berlin, Berlin, Germany.
Gut Pathog. 2025 Jun 22;17(1):48. doi: 10.1186/s13099-025-00721-4.
Arcobacter cryaerophilus is considered an emerging foodborne pathogen and is associated primarily with infectious gastrointestinal disease in humans. However, the underlying pathogenic mechanisms remain poorly understood. Therefore, the aim of the present study was to investigate the pathogenic potential of twelve A. cryaerophilus strains using various in vitro assays in two human colonic cell lines, HT-29/B6 and T84.
All strains tested were able to adhere to and invade into both cell lines, with strain-dependent differences in their adhesion and invasion rates. In addition, two strains showed cytotoxic effects on both cell lines. The ability to disrupt the epithelial barrier function of T84 cell monolayers was shown for two strains by measurement of transepithelial electrical resistance. As structural factors correlate with the barrier dysfunction, immunofluorescence staining of the tight junction domain was performed, and revealed an altered distribution of claudin-5 in infected cells.
The results highlight the strain-dependent pathogenic mechanisms of A. cryaerophilus that may contribute to key symptoms such as diarrhoea. These findings also highlight the importance of further research into the pathogen A. cryaerophilus.
嗜低温嗜冷弧菌被认为是一种新出现的食源性病原体，主要与人类感染性胃肠疾病有关。然而，其潜在的致病机制仍知之甚少。因此，本研究的目的是使用两种人结肠细胞系HT-29/B6和T84，通过各种体外试验来研究12株嗜低温嗜冷弧菌菌株的致病潜力。
所有测试菌株都能够黏附并侵入这两种细胞系，其黏附和侵入率存在菌株依赖性差异。此外，两株菌株对这两种细胞系均表现出细胞毒性作用。通过测量跨上皮电阻，发现两株菌株能够破坏T84细胞单层的上皮屏障功能。由于结构因素与屏障功能障碍相关，因此对紧密连接结构域进行了免疫荧光染色，结果显示感染细胞中claudin-5的分布发生了改变。
这些结果突出了嗜低温嗜冷弧菌菌株依赖性的致病机制，这些机制可能导致腹泻等关键症状。这些发现还突出了对嗜低温嗜冷弧菌病原体进一步研究的重要性。
