Sauberlich H E
Ann N Y Acad Sci. 1975 Sep 30;258:438-50. doi: 10.1111/j.1749-6632.1975.tb29302.x.
Although vitamin C nutritional status in man may be determined on the basis of dietary intake findings and on clinical signs of a dietary deprivation, biochemical measurements represent the most objective approach. Without the availability of a functional biochemical procedure that relates to vitamin C status, information concerning inadequacies in this nutrient has been derived mainly from measuring ascorbate levels in serum (plasma), leukocytes, blood, and urine. The measurement of serum levels of ascorbic acid is the most commonly used and practical procedure for determining vitamin C nutritional status in individuals or population groups. Although leukocyte ascorbate levels provide information concerning the body stores of the vitamin, the measurement is technically more difficult to perform, and, hence, its use is largely confined to clinical situations as an aid in the diagnosis of scurvy. The clinical diagnosis of scurvy can be aided also by information on the urinary levels of ascorbic acid and the use of vitamin C loading or saturation tests. With recognized limitations, ascorbic acid can be measured in biological samples with the use of automated or manual colorimetric and fluorometric procedures. Nutrition surveys conducted in Canada and the United States have indicated vitamin C deficits among certain population groups.
虽然人体维生素C营养状况可根据饮食摄入调查结果和饮食缺乏的临床体征来确定，但生化检测是最客观的方法。在缺乏与维生素C状况相关的功能性生化检测方法时，有关这种营养素不足的信息主要来自于测量血清（血浆）、白细胞、血液和尿液中的抗坏血酸水平。测定血清抗坏血酸水平是确定个体或人群维生素C营养状况最常用且实用的方法。虽然白细胞抗坏血酸水平能提供有关该维生素体内储存情况的信息，但该检测在技术上操作更困难，因此其应用主要局限于临床，辅助坏血病的诊断。尿中抗坏血酸水平信息以及维生素C负荷或饱和试验也有助于坏血病的临床诊断。尽管存在公认的局限性，但可使用自动或手动比色法和荧光法在生物样本中测定抗坏血酸。在加拿大和美国进行的营养调查表明某些人群存在维生素C缺乏情况。
