Lu S H, Wei Y H, Chang L S, Lin A T, Chen K K, Yang A H
Department of Surgery, Veterans General Hospital-Taipei Institute of Clinical Medicine, School of Medicine, Taiwan, Republic of China.
J Urol. 2000 Jan;163(1):225-9.
We correlated ultrastructural changes in mitochondria in the human detrusor with the severity of partial bladder outlet obstruction on urodynamics.
We recruited into the study 52 men with and without bladder outlet obstruction symptoms. The severity of partial bladder outlet obstruction was determined by pressure flow study. Random detrusor biopsy specimens obtained by cystoscopy were fixed immediately and processed for transmission electron microscopic observation. Random areas were photographed for further morphological and morphometric analysis using mitochondrial damage score and stereological principles.
Mitochondrial damage score and mean mitochondrial volume strongly correlated with the urodynamic severity of partial bladder outlet obstruction, while mitochondrial volume density, surface density of the mitochondrial outer membrane and number of mitochondria per unit of cytoplasm area did not significantly correlate with severity.
Detrusor mitochondrial swelling and structural destruction increased with the severity of partial bladder outlet obstruction. These changes may be associated with impaired mitochondrial function and oxidative metabolism after partial bladder outlet obstruction. Detrusor mitochondrial damage may explain voiding dysfunction after partial bladder outlet obstruction develops.
我们将人类逼尿肌线粒体的超微结构变化与尿动力学检查中膀胱出口部分梗阻的严重程度进行关联分析。
我们招募了52名有或无膀胱出口梗阻症状的男性。通过压力流研究确定膀胱出口部分梗阻的严重程度。经膀胱镜获取的随机逼尿肌活检标本立即固定，并进行透射电子显微镜观察。使用线粒体损伤评分和体视学原理对随机区域进行拍照，以进行进一步的形态学和形态计量学分析。
线粒体损伤评分和线粒体平均体积与膀胱出口部分梗阻的尿动力学严重程度密切相关，而线粒体体积密度、线粒体外膜表面密度和单位细胞质面积内的线粒体数量与严重程度无显著相关性。
逼尿肌线粒体肿胀和结构破坏随着膀胱出口部分梗阻严重程度的增加而加重。这些变化可能与膀胱出口部分梗阻后线粒体功能受损和氧化代谢异常有关。逼尿肌线粒体损伤可能解释膀胱出口部分梗阻后出现的排尿功能障碍。
