Bray M, Martinez M, Smee D F, Kefauver D, Thompson E, Huggins J W
Virology Division, USAMRIID, Fort Detrick, MD 21702-5011, USA.
J Infect Dis. 2000 Jan;181(1):10-9. doi: 10.1086/315190.
The efficacy of cidofovir for treatment of cowpox virus infection in BALB/c mice was investigated in an effort to evaluate new therapies for virulent orthopoxvirus infections of the respiratory tract in a small animal model. Exposure to 2(-5)x10(6) pfu of cowpox virus by aerosol or intranasally (inl) was lethal in 3- to 7-week-old animals. One inoculation of 100 mg/kg cidofovir on day 0, 2, or 4, with respect to aerosol infection, resulted in 90%-100% survival. Treatment on day 0 reduced peak pulmonary virus titers 10- to 100-fold, reduced the severity of viral pneumonitis, and prevented pulmonary hemorrhage. The same dose on day -6 to 2 protected 80%-100% of inl infected mice, whereas 1 inoculation on day -16 to -8 or day 3 to 6 was partially protective. Cidofovir delayed but did not prevent the death of inl infected mice with severe combined immunodeficiency. Treatment at the time of tail scarification with vaccinia virus did not block vaccination efficacy.
研究了西多福韦对BALB/c小鼠牛痘病毒感染的治疗效果，以评估在小动物模型中针对呼吸道强毒性正痘病毒感染的新疗法。对3至7周龄的动物通过气溶胶或鼻内（inl）暴露于2(-5)x10(6) 空斑形成单位（pfu）的牛痘病毒是致命的。对于气溶胶感染，在第0、2或4天单次接种100mg/kg西多福韦，可使90%-100%的动物存活。在第0天进行治疗可使肺部病毒峰值滴度降低10至100倍，减轻病毒性肺炎的严重程度，并预防肺出血。在第-6至2天给予相同剂量可保护80%-100%的inl感染小鼠，而在第-16至-8天或第3至6天单次接种则具有部分保护作用。西多福韦可延迟但不能预防严重联合免疫缺陷的inl感染小鼠的死亡。在用痘苗病毒进行尾部划痕接种时进行治疗不会阻碍疫苗接种效果。
