Kawaguchi R, Nagaoka T, Burgess J G, Takeyama H, Matsunaga T
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Plasmid. 1994 Nov;32(3):245-53. doi: 10.1006/plas.1994.1063.
We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.
我们之前已经表明，来自海洋蓝藻聚球藻属Synechococcus sp. NKBG 042902的质粒pSY10的拷贝数取决于生长培养基的盐度。我们在此报告该质粒的完整核苷酸序列（2561 bp）。最长的开放阅读框ORF-B（1.08 kb）位于一个1.6 kb的EcoRI片段上。该ORF编码一个推定的蛋白质，其长度为360个氨基酸残基，与来自集胞藻属Synechocystis sp. 菌株PCC 6803的质粒pCA2.4的复制蛋白有37.8%的同源性，与来自念珠藻质粒pGL2的一个ORF有35.8%的同源性，与来自植物乳杆菌的质粒pC30il的ORF有33.2%的同源性。在ORF-B和其他细菌质粒之间也发现了氨基酸序列的高度保守区域。
