来自瘤胃细菌反刍月形单胞菌HD4的隐蔽质粒的完整核苷酸序列及两个预测开放阅读框的鉴定

Complete nucleotide sequence of a cryptic plasmid from the ruminal bacterium Selenomonas ruminantium HD4 and identification of two predicted open reading frames.

作者信息

Al-Khaldi S F, Evans J D, Martin S A

机构信息

Department of Animal and Dairy Science, The University of Georgia, Athens, Georgia 30602-2771, USA.

出版信息

Plasmid. 1999 Jul;42(1):45-52. doi: 10.1006/plas.1999.1405.

DOI:10.1006/plas.1999.1405

PMID:10413665

Abstract

A cryptic plasmid (pSR1) isolated from Selenomonas ruminantium HD4 was previously cloned into the HindIII site of pBR322 and a restriction map was constructed using HindIII, ClaI, BamHI, and PvuII (S. A. Martin and R. G. Dean, Appl. Environ. Microbiol. 55(12), 3035-3038, 1989). Analysis of the nucleotide sequence of pSR1 revealed two major open reading frames (ORFs) located in the minus strand at different frames. Analysis of ORF-1 revealed that it has 325 amino acids with a predicted MW of 36,588, and ORF-2 has 379 amino acids with a predicted MW of 42,651. The ORF-1 amino acids showed 30 to 32% sequence homology to the hypothetical protein YtqA in Bacillus subtilis and another hypothetical protein in the thermophilic bacterium Aquifex aeolicus. ORF-2 showed limited homology (23%) to the hypothetical protein ICFG in the photosynthetic cyanobacteria Synechocystis PCC6803.

摘要

先前从反刍月形单胞菌HD4中分离出的一种隐蔽质粒（pSR1）被克隆到pBR322的HindIII位点，并使用HindIII、ClaI、BamHI和PvuII构建了限制性酶切图谱（S. A. 马丁和R. G. 迪恩，《应用与环境微生物学》55(12)，3035 - 3038，1989）。对pSR1核苷酸序列的分析揭示了位于负链不同读框中的两个主要开放阅读框（ORF）。对ORF - 1的分析表明，它有325个氨基酸，预测分子量为36,588，而ORF - 2有379个氨基酸，预测分子量为42,651。ORF - 1的氨基酸与枯草芽孢杆菌中的假定蛋白YtqA以及嗜热细菌嗜热栖热菌中的另一种假定蛋白具有30%至32%的序列同源性。ORF - 2与光合蓝细菌聚球藻PCC6803中的假定蛋白ICFG具有有限的同源性（23%）。