培养的鸡肌管和去神经支配的小鼠趾长伸肌合成乙酰胆碱受体。
Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles.
作者信息
Devreotes P N, Fambrough D M
出版信息
Proc Natl Acad Sci U S A. 1976 Jan;73(1):161-4. doi: 10.1073/pnas.73.1.161.
Abstract
Mono-[125I]iodo- alpha - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or 13C-substituted amino acids under conditions of active receptor accumulation, the mono-[125I]iodo-alpha-bungarotoxin-receptor complexes have an increased buoyant density and band in a position of higher density in the gradient relative to a marker of mono-[131I]iodo-alpha-bungarotoxin-receptor complexes extracted from cells preincubated in normal media. It is concluded that the accumulation of receptors on the surfaces of cultured chick myotubes and on the non-synaptic surfaces of extensor digitorum longus muscles following denervation are the result of de novo synthesis.
摘要
从鸡肌管培养物以及从小鼠成年去神经支配的趾长伸肌中提取的单-[125I]碘-α-银环蛇毒素-受体复合物，已在甲泛葡胺-重水梯度中按其浮力密度进行了分带。当培养物或去神经支配的成年肌肉在受体活跃积累的条件下于含有2H或13C取代氨基酸的培养基中预孵育时，单-[125I]碘-α-银环蛇毒素-受体复合物的浮力密度增加，并在梯度中相对于从在正常培养基中预孵育的细胞中提取的单-[131I]碘-α-银环蛇毒素-受体复合物标记物处于更高密度的位置分带。得出的结论是，去神经支配后培养的鸡肌管表面和趾长伸肌非突触表面上受体的积累是从头合成的结果。
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