Pflanzenphysiologisches Institut der Universität Göttingen, Germany.
Plant Physiol. 1978 Aug;62(2):284-6. doi: 10.1104/pp.62.2.284.
The mechanism underlying the sharp increase in activity of nitrate reductase (EC 1.6.6.1) in Chlorella vulgaris forma tertia (strain 211 8k) during the first hour of the 7 hours/5 hours light/dark cycle was investigated. Using the method of density labeling and isopycnic centrifugation, it could be demonstrated that this rapid increase in activity is based on light-mediated activation rather than de novo synthesis of the enzyme. The problematic nature of cycloheximide specificity and models of nitrate reductase activation are discussed.
研究了在 7 小时/5 小时光/暗循环的第一个小时内,小球藻变种(菌株 211 8k)中硝酸还原酶(EC 1.6.6.1)活性急剧增加的机制。使用密度标记和等密度离心的方法,可以证明这种活性的快速增加是基于光介导的激活,而不是酶的从头合成。本文还讨论了环己酰亚胺特异性的问题和硝酸还原酶激活的模型。