Nichols N N, Harwood C S
Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA.
FEMS Microbiol Lett. 2000 Jan 1;182(1):177-83. doi: 10.1111/j.1574-6968.2000.tb08893.x.
An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild-type aer, provided in trans, complemented the aer mutant to an aerotactic response that was stronger than wild-type. The P. putida Aer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, of Escherichia coli. The amino-terminus is similar to redox-sensing regulatory proteins, and the carboxy-terminus contains the highly conserved domain present in chemotactic transducers.
从恶臭假单胞菌PRS2000中克隆出了一个趋氧性基因aer。恶臭假单胞菌aer突变体在毛细管试验中表现出改变的趋氧反应。野生型恶臭假单胞菌聚集在气/液界面处。相比之下，aer突变体不在界面处聚集，而是在距弯月面一定距离处形成一条弥散带。反式提供的野生型aer将aer突变体互补至比野生型更强的趋氧反应。恶臭假单胞菌Aer序列在其全长上与大肠杆菌的趋氧性（能量趋化性）信号转导蛋白Aer相似。氨基末端类似于氧化还原感应调节蛋白，羧基末端包含趋化性转导器中存在的高度保守结构域。
