Rincé A, Tangney M, Fitzgerald G F
National Food Biotechnology Centre, University College, Cork, Ireland.
FEMS Microbiol Lett. 2000 Jan 1;182(1):185-91. doi: 10.1111/j.1574-6968.2000.tb08894.x.
Bacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage sk1, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages.
噬菌体712是一种小型等轴头噬菌体，对乳球菌流产感染机制AbiF敏感。其29.6 kb的DNA基因组通过限制性图谱分析和转录分析进行了表征。在含有AbiF的乳酸乳球菌中构建对AbiF作用不敏感的乳球菌噬菌体sk1基因文库，结果鉴定出一个324 bp的DNA片段，该片段降低了流产感染机制对噬菌体712的影响。对该区域的分析提供了证据，表明AbiF的作用与小型等轴头噬菌体的粘性末端有关。对包含噬菌体712基因组中间操纵子和粘性末端的3.2 kb片段进行序列分析，使得能够将噬菌体712基因组的这一部分与其他四种小型等轴头噬菌体的等效序列进行比较。
