参与对乳酸乳球菌流产感染机制AbiD1敏感性的噬菌体操纵子。
Phage operon involved in sensitivity to the Lactococcus lactis abortive infection mechanism AbiD1.
作者信息
Bidnenko E, Ehrlich D, Chopin M C
机构信息
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
出版信息
J Bacteriol. 1995 Jul;177(13):3824-9. doi: 10.1128/jb.177.13.3824-3829.1995.
Abstract
Phage bIL66 is unable to grow on Lactococcus lactis cells harboring the abortive infection gene abiD1. Spontaneous phage mutants able to grow on AbiD1 cells were used to study phage-Abi interaction. A 1.33-kb DNA segment of a mutant phage allowed growth of AbiD1s phages in AbiD1 cells when present in trans. Sequence analysis of this segment revealed an operon composed of four open reading frames, designated orf1 to orf4. The operon is transcribed 10 min after infection from a promoter presenting an extended -10 consensus sequence but no -35 sequence. Analysis of four independent AbiD1r mutants revealed a different point mutation localized in orf1, implying that this open reading frame is needed for sensitivity to AbiD1. However, the sensitivity is partly suppressed when orf3 is expressed in trans on a high-copy-number plasmid, suggesting that AbiD1 acts by decreasing the concentration of an available orf3 product.
摘要
噬菌体bIL66无法在携带流产感染基因abiD1的乳酸乳球菌细胞上生长。利用能够在AbiD1细胞上生长的自发噬菌体突变体来研究噬菌体与AbiD1的相互作用。当一个突变噬菌体的1.33 kb DNA片段以反式存在时，可使AbiD1噬菌体在AbiD1细胞中生长。对该片段的序列分析揭示了一个由四个开放阅读框组成的操纵子，命名为orf1至orf4。感染后10分钟，该操纵子从一个具有延伸的-10共有序列但无-35序列的启动子开始转录。对四个独立的AbiD1r突变体的分析揭示了一个位于orf1中的不同点突变，这意味着该开放阅读框是对AbiD1敏感所必需的。然而，当orf3在高拷贝数质粒上以反式表达时，敏感性会部分受到抑制，这表明AbiD1通过降低可用的orf3产物的浓度起作用。
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