Kashiba H, Senba E
Department of Physiology, Kansai College of Oriental Medicine, Sennan, Osaka, Japan.
Neuroreport. 1999 Nov 26;10(17):3561-5. doi: 10.1097/00001756-199911260-00018.
Anterograde transport of BDNF is enhanced by axotomy in the rat sciatic nerve. However, the changes in BDNF gene expression in dorsal root ganglion (DRG) neurons after axotomy are not known. We examined this issue using in situ hybridization histochemistry. BDNF mRNA was detected in 35-40% of DRG neurons (L5) of control rats. Most of these neurons are small. BDNF gene expression in these neurons was down-regulated after application of capsaicin to the sciatic nerve. Transection of the sciatic nerve induced the up-regulation of BDNF mRNA. The intensely labeled neurons were mainly large and immunoreactive for neuropeptide Y. These results suggest that up- and down-regulation of BDNF gene expression in distinct subgroups of rat DRG neurons are caused by damage to the peripheral nerve.
大鼠坐骨神经轴突切断术可增强脑源性神经营养因子（BDNF）的顺行运输。然而，轴突切断术后背根神经节（DRG）神经元中BDNF基因表达的变化尚不清楚。我们使用原位杂交组织化学技术研究了这个问题。在对照大鼠的35%-40%的DRG神经元（L5）中检测到BDNF mRNA。这些神经元大多数较小。将辣椒素应用于坐骨神经后，这些神经元中的BDNF基因表达下调。坐骨神经横断诱导BDNF mRNA上调。标记强烈的神经元主要是大神经元，对神经肽Y有免疫反应。这些结果表明，大鼠DRG神经元不同亚组中BDNF基因表达的上调和下调是由外周神经损伤引起的。
