通过同位素比率分析测定的乳酸菌在葡萄糖/柠檬酸盐共发酵中的代谢通量。
Metabolic flux in glucose/citrate co-fermentation by lactic acid bacteria as measured by isotopic ratio analysis.
作者信息
Goupry S, Croguennec T, Gentil E, Robins R J
机构信息
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, CNRS ESA 6006, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, 44322, Nantes, France.
出版信息
FEMS Microbiol Lett. 2000 Jan 15;182(2):207-11. doi: 10.1111/j.1574-6968.2000.tb08896.x.
The flux of carbon into lactic acid, diacetyl and acetoin during the co-metabolism of glucose and citrate by Lactococcus lactis subsp. lactis biovar. diacetylactis has been determined using natural abundance isotopic ratio analysis. During fermentation in the conditions used (glucose, 27.8 mM; citric acid, 13.9 mM; initial pH 6.2-6.4, anaerobic) it is shown that approximately 65% of the carbon source used for the aroma compounds is derived from the carbohydrate. Equally, citrate contributes approximately 30% of the carbon recovered in lactic acid. Thus, there is no evidence for a metabolic separation of the catabolism of these two carbon sources.
利用自然丰度同位素比率分析，测定了乳酸乳球菌乳亚种双乙酰变种在葡萄糖和柠檬酸盐共代谢过程中碳流入乳酸、双乙酰和乙偶姻的通量。在所使用的发酵条件下（葡萄糖，27.8 mM；柠檬酸，13.9 mM；初始pH 6.2 - 6.4，厌氧），结果表明，用于生成香气化合物的碳源中约65%来自碳水化合物。同样，柠檬酸盐对乳酸中回收碳的贡献约为30%。因此，没有证据表明这两种碳源的分解代谢存在代谢分离。
Zotero 插件