Whitehead T R, Cotta M A
Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, USA.
FEMS Microbiol Lett. 2000 Jan 15;182(2):237-40. doi: 10.1111/j.1574-6968.2000.tb08901.x.
Streptococcus bovis has been identified as a causative agent in humans for a variety of diseases, including endocarditis, meningitis, and septicemia. Identification of S. bovis strains of human origin in clinical settings has been problematic due to variations in biochemical tests as compared to ruminal strains of S. bovis, and other streptococcal species. DNA-DNA hybridization with chromosomal DNA from various S. bovis strains indicates that strains of human origin are different from those of ruminal origin. Specific probes have been designed from S. bovis 16S rDNA gene sequences that differentiate strains of human and ruminal origin by direct hybridization and PCR analyses. These techniques now allow for rapid identification of S. bovis strains for clinical and other scientific investigations.
牛链球菌已被确认为人类多种疾病的病原体，包括心内膜炎、脑膜炎和败血症。由于与牛链球菌瘤胃菌株及其他链球菌物种相比，生化试验存在差异，因此在临床环境中鉴定人类来源的牛链球菌菌株一直存在问题。对各种牛链球菌菌株的染色体DNA进行DNA-DNA杂交表明，人类来源的菌株与瘤胃来源的菌株不同。已根据牛链球菌16S rDNA基因序列设计了特异性探针，可通过直接杂交和PCR分析区分人类和瘤胃来源的菌株。这些技术现在可用于临床和其他科学研究中快速鉴定牛链球菌菌株。
