问号钩端螺旋体S10-spc-α操纵子的特性分析。

Characterization of the Leptospira interrogans S10-spc-alpha operon.

作者信息

Zuerner R L, Hartskeerl R A, van de Kemp H, Bal A E

机构信息

Zoonotic Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 70, 2300 Dayton Road, Ames, IA 50010, USA.

出版信息

FEMS Microbiol Lett. 2000 Jan 15;182(2):303-8. doi: 10.1111/j.1574-6968.2000.tb08912.x.

DOI:10.1111/j.1574-6968.2000.tb08912.x

PMID:10620683

Abstract

A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and alpha operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-alpha cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution.

摘要

对问号钩端螺旋体的一个核糖体蛋白基因簇进行了表征。该基因座与大肠杆菌的S10、spc和α操纵子同源。对问号钩端螺旋体RNA的分析表明，该簇内的核糖体蛋白基因是共转录的，从而形成一个操纵子。通过引物延伸确定了两个转录起始位点，位于该簇中第一个基因fus的上游，该区域的序列在大肠杆菌中具有启动子活性。转录在该簇中最后一个基因rplQ之后的一个预测茎环结构附近终止。这些数据表明，fus上游的两个启动子指导这个17.5 kb核糖体蛋白基因簇的转录。将问号钩端螺旋体的S10-spc-α簇与伯氏疏螺旋体和梅毒螺旋体的同源基因座进行比较，提供了证据表明该基因组区域在螺旋体进化过程中经历了几次重排。